Fig. 2

High chromatin accessibility and gene expression by comparing different sets of tumor samples through analysis of ATAC-seq and RNA-seq datasets identified GSTM1 as a putative biomarker. a Venn diagrams of overlapping datasets (tumor vs. normal and short-survival vs. long-survival) showed that 11 genes had induced gene expression and increased chromatin accessibility in their promoters. b Heatmap analysis of gene expression showed that GSTM1 ranked on the top of genes with the highest expression through comparing different groups. Heatmap of RNA-seq data by counts per million (CPM) of each gene was shown. c Heatmap analysis of chromatin accessibility of genes showed that GSTM1 had the highest chromatin accessibility of promoter regions through comparing different groups. Heatmap of ATAC-seq data by counts per million (CPM) of each gene was shown. d Comparison of representative ATAC-seq gene tracks in the GSTM1 locus from short-survival, long-survival, and normal tumor samples. Red-shaded area showed the GSTM1 tracks from different samples. Gene tracks of RBM15 locus on chromosome 1 from different samples were shown as controls