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Fig. 5 | Clinical Epigenetics

Fig. 5

From: High-throughput and affordable genome-wide methylation profiling of circulating cell-free DNA by methylated DNA sequencing (MeD-seq) of LpnPI digested fragments

Fig. 5

Comparison of cfDNA methylation profiles from HBDs and CRLM patients before and after surgery. A Principal components were calculated for cfDNA methylation profiles of 9 HBDs (4 males and 5 females; in black) and a pre- (BL; in red) and post-operative (+ 5d; in orange) sample of 8 CRLM patients. PC1 and PC2, with the explained variances, are shown on the x-axis and y-axis, respectively. Shapes represent patients, where each icon represents 1 cfDNA sample. B Unsupervised hierarchical clustering of methylation profiles again shows a distinct cluster for pre-operative CRLM samples (red). HBDs (black) and post-operative CRLM samples (orange) show more similar methylation profiles. C Obtained methylation profiles were summarized in a genome-wide Z-score as an overall measure for the deviation of the sample from the average normal methylation profile in HBDs. Pre-operative genome-wide Z-scores are depicted in grey and post-operative genome-wide Z-scores in black

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Clinical Epigenetics

ISSN: 1868-7083

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