Prenatal and postnatal traffic pollution exposure, DNA methylation in Shank3 and MeCP2 promoter regions, H3K4me3 and H3K27me3 and sociability in rats’ offspring

Table 3 Average methylation ratios (%) in Shank3 and MeCP2 promoter regions (n = 10 per group)

	Gender	EL	EH	PL	PH	Timing P value	Site P value	Timing × site P value
MeCP2_01	F	31.3 ± 2.0	31.6 ± 1.3	32.3 ± 1.1	32.6 ± 1.2	0.040 (0.037)*	0.481 (0.475)	0.942
MeCP2_01	M	0.88 ± 0.17	0.84 ± 0.12	1.21 ± 0.33	1.14 ± 0.25	< 0.001 (< 0.001)***	0.447 (0.441)	0.786
MeCP2_02	F	34.2 ± 1.4	34.3 ± 1.7	36.7 ± 1.7	36.4 ± 1.9	< 0.001 (< 0.001)***	0.903 (0.902)	0.662
MeCP2_02	M	0.99 ± 0.22	0.97 ± 0.20	1.54 ± 0.38	1.41 ± 0.44	< 0.001 (< 0.001)***	0.456 (0.451)	0.626
Shank3_01	F	1.60 ± 0.29	1.74 ± 0.17	1.81 ± 0.19	1.71 ± 0.17	0.199 (0.214)	0.730 (0.739)	0.065
Shank3_01	M	1.66 ± 0.23	1.67 ± 0.17	1.81 ± 0.02	1.76 ± 0.31	0.117 (0.113)	0.791 (0.788)	0.705
Shank3_02	F	0.93 ± 0.05	0.97 ± 0.06	1.08 ± 0.04	1.14 ± 0.08	< 0.001 (< 0.001)***	0.013 (0.013)*	0.473
Shank3_02	M	0.93 ± 0.05	0.99 ± 0.09	1.06 ± 0.08	1.09 ± 0.06	< 0.001 (< 0.001)***	0.041 (0.039)*	0.536
Shank3_03	F	1.66 ± 0.13	1.65 ± 0.09	2.48 ± 0.20	2.53 ± 0.20	< 0.001 (< 0.001)***	0.685 (0.682)	0.612
Shank3_03	M	1.67 ± 0.11	1.70 ± 0.18	2.43 ± 0.26	2.46 ± 0.06	< 0.001 (< 0.001)***	0.548 (0.543)	0.984

F: female; M: male. L: green space; H: crossroads. E: exposure from embryonic day 0 (E0) to embryonic day 20 (E20); P: exposure from postnatal day (PND) 1 to postnatal day 21. *P < 0.05, **P < 0.01, ***P < 0.001

ISSN: 1868-7083