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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Epigenetic targeting of the ACE2 and NRP1 viral receptors limits SARS-CoV-2 infectivity

Fig. 3

Pretreatment with VPA reduces the viral infection mediated by SARS-CoV-2. a Schematic of the experiment to determine the prophylactic effect of VPA on viral production. HK-2 and Huh-7 cells were treated with VPA at 4, 8, and 16 mM concentrations, or left untreated (control cells). At 24 h after treatment, culture medium was replaced by fresh medium without VPA. Cells were infected with the different viruses independently and the supernatants were collected at 24 and 48 h post-infection (hpi) for virus titration by plaque assay. Viruses analyzed were SARS-CoV-2 (MOI 0.5, b), VSV-GFP (MOI 0.01, c), and HCoV-229E (MOI 0.1, d). The latter was assayed only in Huh-7 cells. Virus titers were determined by a lysis plaque assay and represented in absolute numbers as plaque-forming units per ml (PFU/ml). Data are represented as the mean ± SD of three independent experiments. *p < 0.05

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