Fig. 5

UNC-0379 treatment causes similar nucleolar alterations in wild-type and p53 depleted-myeloma cells. a Immunofluorescence microscopy of nucleolin localization and DNA (DAPI staining) in XG7 cells depleted or not for p53 and treated with 3 μM of UNC-0379 or the vehicle DMSO (mock) for 24 h. Scale bar: 10 μm. Visual quantification of percentage of cells with nucleolin diffusion out of the nucleolus. A minimum of 300 cells were quantified per condition. N = 3. Statistical significance between conditions was assessed using Student paired t test (*p value < 0.05, **p value < 0.01, ns: not significative). b Analysis of RNA synthesis by 5 min 5-Ethynyl Uridine (EU) pulse-labeling in XG7 cells depleted or not for p53 and treated with 3 μM of UNC-0379 or the vehicle DMSO (mock) for 24 h. Scale bar: 10 μm. EU intensity was quantified using CellProfiler “Human cell” pipeline. A minimum of 200 cells were quantified per condition. Graph shows the quantification of one representative experiment of 3