Fig. 4
UNC-0379 toxicity is independent of p53. a Comparison of UNC-0379 IC50 (µM) according to HMCLs TP53 status. b Immunoblot analysis of indicated proteins in total lysates from UNC-0379-treated (5 µM) or untreated XG7-shControl and XG7-shp53 HMCLs. β-actin and H2A.X were used as loading controls. c First bar plot represents cell cycle of control and 48 h UNC-0379-treated (5 µM) XG7-shp53 analyzed by flow cytometry using DAPI, BrdU incorporation and labelling with an anti-BrdU antibody. Results are representative of three independent experiments. Second bar plot represents apoptosis induction in control and UNC-0379-treated (5 µM) XG7-shControl and XG7-shp53 analyzed by AnnexinV-PE staining and flow cytometry after 96 h treatment. Data shown are mean values ± SD of 4 separate experiments. Statistical analysis was done with a paired t test. *Indicates a significant difference compared to control cells using a Wilcoxon test for pairs (p ≤ 0.05)