Fig. 3

The biological function of the genes differentially methylated in SYM and ASYM plaques. a and d GO analysis of hypomethylated and hypermethylated genes in SYM compared with ASYM plaques using ClueGO and DAVID (n = 6 for SYM plaques and n = 8 for ASYM plaques). Nodes are linked based on their kappa score levels (> 0.03). The names of each group are labeled. The node size demarcates the enrichment significance of each term. Functionally related groups are partially overlapped. b and e Histogram of the most enriched biological function-specific terms of hypomethylated and hypermethylated genes. The length of the bar is the percentage of hypomethylated genes in each functional term (GO database), and the corresponding gene number is presented beside the bar. Those terms are sorted in the order of p value. c and f Overview pie-chart of the hypomethylated and hypermethylated genes show enriched functional groups, the percentage of certain groups were obtained from the GO analysis. SYM, symptomatic; ASYM, asymptomatic; GO, gene ontology; and DAVID, the database for annotation, visualization and integrated discovery