Fig. 2

Increased phenotypic alterations in developing embryos following early pre-implantation alcohol exposure. a Percentage of E10.5 embryos with phenotypic alterations (Ctrl: 2%; n = 2/108, EtOH-exposed: 19%; n = 29/152, ****p < 0.0001; chi-square test). b Classification and proportion of phenotypic alterations observed in EtOH-exposed embryos. c Examples of primary phenotypic alterations observed in EtOH-exposed embryos. Views from both side of the embryo and zoom on the head for control (i, vi, xi) and EtOH-exposed embryos with severe developmental delays (ii, vii, xii), brain malformations (iii, viii, xiii, iv, ix, xiv) and no apparent defects (v, x, xv). d Number of embryos with alterations and number of litters with at least one affected embryo. ***p < 0.001; chi-square test