Epigenetic age is associated with baseline and 3-year change in frailty in the Canadian Longitudinal Study on Aging

Table 2 A description of the methodology used to derive each epigenetic clock employed in the current study

Clock	Classification	Normalization approach	CpG availability	Derivation method
Hannum [16]	Age-trained	Noob [57]	65 of 71	R software: ENmix (methyAge function) [64]
Horvath [15]	Age-trained	Noob [57]	334 of 353	Online software: http://dnamage.genetics.ucla.edu/
Lin [30]	Age-trained	Preprocessillumina [57]	97 of 99	Used published weights [30]
Yang [31]	Mitotic clock	BMIQ [65]	354 of 385	R software [31]
Dunedin PoAm [20]	Phenotype	BMIQ [65]	46 of 46	R software: DunedinPoAm38 [20]
GrimAge [22]	Phenotype/mortality	Noob [57]	1030 of 1030*	Online software: http://dnamage.genetics.ucla.edu/
PhenoAge [21]	Phenotype/mortality	Noob [57]	513 of 513	Online software: http://dnamage.genetics.ucla.edu/
Zhang [32]	Mortality	Preprocessillumina [57]	8 of 10	Used published weights [32]

^*GrimAge CpGs have not been released, so the availability of sites for the current study is assumed given that the algorithm was designed to be compatible with the EPIC 850 K array [22]

ISSN: 1868-7083