Table 2 A description of the methodology used to derive each epigenetic clock employed in the current study
Clock | Classification | Normalization approach | CpG availability | Derivation method |
|---|---|---|---|---|
Hannum [16] | Age-trained | Noob [57] | 65 of 71 | R software: ENmix (methyAge function) [64] |
Horvath [15] | Age-trained | Noob [57] | 334 of 353 | Online software: http://dnamage.genetics.ucla.edu/ |
Lin [30] | Age-trained | Preprocessillumina [57] | 97 of 99 | Used published weights [30] |
Yang [31] | Mitotic clock | BMIQ [65] | 354 of 385 | R software [31] |
Dunedin PoAm [20] | Phenotype | BMIQ [65] | 46 of 46 | R software: DunedinPoAm38 [20] |
GrimAge [22] | Phenotype/mortality | Noob [57] | 1030 of 1030* | Online software: http://dnamage.genetics.ucla.edu/ |
PhenoAge [21] | Phenotype/mortality | Noob [57] | 513 of 513 | Online software: http://dnamage.genetics.ucla.edu/ |
Zhang [32] | Mortality | Preprocessillumina [57] | 8 of 10 | Used published weights [32] |