Fig. 7

MEST transcript illustration and bisulfite sequencing of two potentially regulatory CpG-rich areas. A Island 1 is located in a typical promoter zone with the capacity to regulate the closest transcripts and possibly distant ones (Cr 7: 130 126 018—130 126 801 identified in A2780-R). A region of 529 bp in length was sequenced. Island 2, which is located in the gene body, could fit within a promoter region for transcripts 201, 9, 1, 5, 11 and 12 and possibly for transcript 17 (Cr7: 130 130 377–130 132 422, identified in A2780-R). An area of 429 bp in length with 20 CpG positions was selected to be sequenced. B Island 1. Representation of a sequence fragment of the ​​bisulfite-modified DNA MEST gene area from sensitive and resistant tumor lines A2780, normal ovarian tissue from patients undergoing sex change, DNA from peripheral blood mononuclear cells (PBMCs) and tumors from fresh tissue. All samples were sequenced with the antisense primer. C Island 2. Representation of a sequence fragment of the ​​bisulfite-modified DNA MEST gene area. In addition to the samples sequenced for the evaluation of area 1, we added a fallopian tube sample and tumor 754. PBMCs, tumors 568, 569 and 5T were not evaluated for this region. All samples were sequenced with the sense primer. Methylated positions are indicated by a blue asterisk