Fig. 4

DNA methylation levels and functional characterization of the DMRs associated with genes showing spermatogenesis-regulated expression. Box plots show the distribution of the methylation values for CTR (teal, n = 4) and CZ (purple, n = 4) (Additional file 1: Table S8). The overlaps of each DMR with specific genomic features are shown: exons (yellow), introns (light-blue), lncRNAs (grey), promoters (orange), UMRs (unmethylated regions, green), LMRs (low-methylated regions, dark-blue) and GeneHancer “double-elite” regulatory regions (pink). The 13 genes in bold were shown to be differentially expressed between CTR and CZ (see Fig. 5)