Fig. 1

Testicular germ cell samples selection for whole genome bisulfite sequencing. a Schematic representation of the experimental design. b Box plot showing the methylation values of H19, MEST, DDX4 and XIST measured using deep bisulfite sequencing (DBS) of the supernatant fraction at day 3–4 of culture for the normal controls (CTR, teal, n = 4) and the cryptozoospermic (CZ, purple, n = 4) samples. No significant difference was found in the methylation values of the four genes between the two groups. c Box plot showing the results of the ploidy analysis of the day 0 single-cell suspension of the normal controls (CTR, teal, n = 4) and the cryptozoospermic (CZ, purple, n = 4) samples used for whole genome bisulfite sequencing (WGBS). d Stacked bar plots showing the percentages of tubules containing germ cells (most advanced germ cell type shown), only Sertoli cells, or tubular shadows in each biopsy from which the samples for WGBS were prepared. e Box plot showing the results of the ploidy analysis of the supernatant fraction at day 3–4 of culture for the normal controls (CTR, teal, n = 4) and the cryptozoospermic (CZ, purple, n = 4) samples. No significant difference was found in the cellular composition of the supernatant fraction between the two groups