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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Genome-wide DNA methylome analysis identifies methylation signatures associated with survival and drug resistance of ovarian cancers

Fig. 5

Cisplatin, azacytidine, decitabine, and thioguanine exerted inhibitory effects on ovarian cancer cell lines in vitro. a XTT assay demonstrated the reduction of cell viability on different ovarian cancer cell lines upon treatment with different concentrations of cisplatin, azacytidine, decitabine, and thioguanine for 48 h. b Flow cytometry on Annexin V-PI staining showed the effect of cisplatin (10 µM), azacytidine (20 µM), decitabine (80 µM) and thioguanine (80 µM) on the induction of apoptosis in A2780cp and A2780s cells after a 48-h treatment. Data analysis was performed with CytExpert software. Populations of cells distributed in different quadrants are presented in different colors. c Flow cytometry on PI staining revealed the effect of cisplatin (2.5 µM), azacytidine (5 µM), decitabine (20 µM), and thioguanine (20 µM) on interruption of cell cycle progression on A2780cp and A2780s cells after a 48-h treatment. Data analysis was performed with Modfit LT software. Populations of cells distributed in different phases are presented in different shaded peaks. For all, n = 3 technical replicates per sample. Data are represented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.0001 (Student’s t test)

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