Fig. 3

EOC with higher global DNA methylation levels has higher recurrence and poor overall survival (OS). a Heatmap visualization constructed using k-means clustering of differential methylation positions (DMPs:301) (5% false discovery rate) with at least a 20% difference in the methylation level between “alive without disease” and “alive with disease plus died” groups across 80 EOC cases. b Volcano plot showing the distribution of 180 hypermethylated DMPs (red) and 121 hypomethylated DMPs (blue) with FDR-adjusted P < 0.05 and difference in β value (|logFC|> 0.2). Dashed lines represent cutoffs for significance. The most differentially regulated genes of hypomethylated and hypermethylated sites are marked. Kaplan–Meier estimate of OS using the methylation signature of genes c CLDN16 and PIK3C2G) and d WWP1, FNDC3B, DISP1, NRIP1, PDE4D and CLDN12