Fig. 6
From: SETD2-mediated epigenetic regulation of noncanonical Wnt5A during osteoclastogenesis
K/BxN serum-induced arthritis effects on SETD2 and Wnt signaling molecules. a Ankle morphology (arrowhead) after induced arthritic development. b Measured ankle thickness is shown graphically after arthritic development. Star (*) indicates p < 0.05. c Relative gene expressions of Setd2, Wnt5a, and Wnt3a were determined in bone marrow monocytes after induction of arthritis in mice using qRT-PCR keeping β-Actin as an internal control. d Immunofluorescence analysis of SETD2, WNT5A, and WNT3A protein expression in bone marrow monocytes after arthritic development. e ChIP-qRT-PCR analysis of H3K36me3, H3K27me1, H3K27me3, and H3K27Ac enrichment on the Wnt5a gene in bone marrow monocytes after arthritic development. ChIP with IgG rabbit served as a negative control