Fig. 4

Transcriptional regulations of Wnt5a via SETD2. a UCSC genome browser analysis of RAW 264.7 cell line. The image is showing the enrichment pattern of H3K36me3, H3K27Ac, and H3K27me3 marks on the Wnt5a/b gene in wild type untreated RAW 264.7 cells. Red star (*) marks are specific regions that were analyzed by ChIP-qRT-PCR on the Wnt3a gene. b Quantitative RT-PCR analysis of ChIP-DNA for H3K36me3, H3K27me1, H3K27me3, and H3K27Ac enrichment on the Wnt5a gene using specific primers designed from the intragenic and upstream region of TSS in osteoclast differentiated cells. c ChIP was performed for H3K36me3, H3K27me1, H3K27me3, and H3K27Ac marks on the Wnt5agene in SETD2 knocked down and overexpressed cells. ChIP with IgG rabbit served as a negative control for all ChIP analyses