Skip to main content

Table 3 Added explanatory power by early-PN-induced altered DNA methylation in predicting more emotional/behavioral problems with early-PN

From: Differential DNA methylation by early versus late parenteral nutrition in the PICU: a biological basis for its impact on emotional and behavioral problems documented 4 years later

Outcome assessed at 4-years follow-up Non-linear regression models
Without randomized intervention With randomized intervention
R2a Fold-increase in R2b R2c Fold-increase in R2d
Internalizing problems as reported by parents/caregivers 0.618 2.026 0.619 1.851
Externalizing problems as reported by parents/caregivers 0.603 1.813 0.603 1.710
Total problems as reported by parents/caregivers 0.613 1.868 0.614 1.771
  1. All models were evaluated in 100 bootstrapped replicates. Baseline risk factors included age, center, race [7], gender, geographic origin [7], language, history of malignancy, diabetes, a predefined “syndrome” (Additional file 2), diagnosis and severity of illness (PIM3 and PeLOD) [9, 10], and risk of malnutrition (STRONGkids) [8]. R2 for non-linear models computed according to Rubin’s rules
  2. PN parenteral nutrition
  3. aOptimism-corrected R2 of multivariable non-linear random forest regression models for outcomes assessed at 4-years follow-up including 37 differentially methylated CpG-sites and baseline risk factors
  4. bOptimism-corrected R2 fold-increase with respect to multivariable non-linear regression models including baseline risk factors only
  5. cOptimism-corrected R2 of multivariable non-linear random forest regression models for outcomes assessed at 4-years follow-up including 37 differentially methylated CpG-sites, baseline risk factors and the randomized intervention
  6. dOptimism-corrected R2 fold-increase with respect to multivariable non-linear random forest regression models only including baseline risk factors and the randomized intervention