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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Spatial epi-proteomics enabled by histone post-translational modification analysis from low-abundance clinical samples

Fig. 3

Quantification of histone PTMs from mouse pancreas laser microdissected samples. a Schematic representation of the protocols used to isolate histones from laser microdissected OCT or FFPE samples. b List of differentially modified histone peptides identified and quantified from manually macrodissected areas corresponding to approximately 500,000 or 100,000 cells, or laser microdissected areas (20,000, 5000, 2500 and 1000 cells) processed using the in-gel PRO-PIC digestion protocol. Four samples—comprising both FFPE and OCT tissues-were analyzed for each condition, with the exception of the 100,000 condition, which was acquired in duplicate. White spaces indicate that the peptide was not quantified. c Correlation matrix, based on Pearson's correlation coefficients (shown in the matrix) of log2 (L/H ratios) values for differentially modified histone peptides in the whole slice and LMD samples shown in (b)

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