Fig. 4

The promoter Methylation Classifier for Androgen receptor activity (MCA) signature in relation to tumor and patient characteristics. A Heat map showing the 4580 CpG sites in the MCA signature which correlated to AR activity score (corr > 0.4 or <  − 0.4, StDev > 0.15) in 66 bone metastases from PC. Primary tumors (n = 12) and non-malignant prostate tissue (n = 12) are shown as reference. MCA negative and MCA positive metastasis are marked in the figure. Colored bars show sample characteristics with respect to patient treatment status, tissue composition, MetA–C class, and AR copy number. Integrated expression analysis and enriched pathways for genes in cluster A–E are shown in Table 2. B AR activity scores of MCA positive and MCA negative bone metastasis. C Proliferation scores of the MCA positive and MCA negative bone metastasis samples. D Serum PSA levels of MCA positive and MCA negative patients with metastases. E Kaplan Meier survival analysis of MCA positive and MCA negative patients with metastases. The AR and proliferation scores are defined and calculated as described in the “Material and methods” section