Fig. 6
From: Oxygen concentration affects de novo DNA methylation and transcription in in vitro cultured oocytes
CGI methylation under four different conditions using oocyte in vitro model. a Bean plots representing total methylation in IV in vivo, IV 20% O2, MAO, LiCl, MAO + LiCl-grown oocytes and Kdm1a, Kdm1b knock-out and WT M2 oocytes, (χ2 test, p < 0.05). b Percentage of methylation at CGIs between in vivo and cultured oocytes (for IV in vivo n = 5707, IV 20% O2 CON n = 5713, MAO n = 5713, LiCl n = 5713, MAO + LiCl n = 5713, χ2 test, p < 0.05), c Heat map of the CGIs over 40% of methylation in at least one sample between the conditions, (n = 1138; χ2 test, p < 0.05). d Distribution of CGIs over 40% of methylation in at least one sample (n = 1138) located overlapping promoter, intragenic, and intergenic regions (χ2 test, p < 0.05). e Heatmap representing the quantitation of the methylation difference between the IV 20% O2 CON compared to each cultured in vitro oocytes of the 23 igDMRs (χ2 test, p < 0.05)