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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Oxygen concentration affects de novo DNA methylation and transcription in in vitro cultured oocytes

Fig. 5

DNA methyl transferase expression in grown oocytes. a Dnmt1, Dnmt3a, and Dnmt3l expression compared to GV. *p > 0.05 Student’s t test GV compared to cultured oocytes. b Immunostaining of Dnmt3a in different size oocytes.  c Quantification of immunofluorescence intensity as seen in (b). Corrected total cell fluorescence (CTCF) was calculated for each signal then normalised to DAPI CTCF. Bars represent mean values, and error bars show SEM (2-way ANOVA test; *p < 0.05; **p < 0.01, ***p < 0.001)

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