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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Oxygen concentration affects de novo DNA methylation and transcription in in vitro cultured oocytes

Fig. 1

Characterisation of follicle-free cultured oocytes. a Schematic representation of the in vitro oocyte culture model. The time of collection of cultured oocytes under 20% O2 was day 21 (14 days after start of the culture), and for the oocytes grown under 5% O2, it was on day 16 (9 days after the starting the culture). b Bright-field photomicrograph of in vitro-grown oocyte cultured for 9 days under 5% O2; *indicates oocyte of 70 µm diameter, scale bar 35 µm. c Graph of the proportions of oocytes of the various size categories after 14 days of culture (20% O2) and 9 days of culture (5% O2). Combined data from 7 independent cultures. ****p < 0.001 (ANOVA test). d Contingency graph summarising the growth of the cultured oocytes under 20% O2 and 5% O2. Combined data from 7 independent cultures. e DAPI staining of GV-like oocytes grown in 20% O2 and 5% O2. Pie charts summarising the number of IV oocytes grown under 20% O2 and 5% O2 classified as non-surrounding nucleus (NSN) and surrounding nucleus (SN) (3 independent oocyte cultures for both conditions, total of oocytes n = 68 for 5% O2 and n = 62 for 20% O2, and n = 31 GV oocytes aged of 23–25 days old). Scale bar 20 µm

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