Skip to main content
Fig. 1 | Clinical Epigenetics

Fig. 1

From: Identification of DNA methylation biomarkers for risk of liver metastasis in early-stage colorectal cancer

Fig. 1

Flowchart for identifying prognostic and predictive DMRs. Targeted bisulfite sequencing was performed on a total of 81 samples containing 59 primary CRC tumor samples (11 stage I, 38 stage II, 10 stage IV), as well as 11 tumor-adjacent samples and 11 metastatic tumor samples. Aim 1. prognostic marker selection: DMRs were identified between the stage II unfavorable group (17 samples) and the stage II favorable prognosis group (21 samples). Jonckheere trend test was performed on 70 samples including 11 tumor adjacent and 59 primary samples. 213 DMRs were selected as the final prognostic markers. Aim 2: LIM predictive marker selection: a LASSO-based LOOCV was applied to a training cohort of 59 primary CRC tumor samples to identify a final selection of 23 DMR markers. These 23 markers were then validated by a dataset of 6 LIM and 5 LUM tumor samples. LIM: liver metastasis; LUM: lung metastasis

Back to article page

Clinical Epigenetics

ISSN: 1868-7083

Contact us