Fig. 8

Effect of Vorinostat treatment on global chromatin accessibility and Pt-adduct formation. a TapeStation trace and b gel images showing, respectively, peaks and bands corresponding to mono-, di- and tri-nucleosome-associated DNA fragments following MNase digestion. Samples were treated with 20 µM Vorinostat or vehicle for 24 h. c Quantification of percent integrated area associated with mono-, di-, trinucleosomal and larger fragments following 24 h treatment with Vorinostat or vehicle, from b. There was a significant difference in integrated area between the vehicle and Vorinostat treated cells. Mono-, p = 0.007, di-, p = 0.006, tri-p = 0.012, larger, p = 0.007, t-test. c Levels of DNA platination as measured by ICP-MS, when PEO4 cells were treated with 0. 16 or 32 µM cisplatin for 5 h following 24 h pre-treatment with 20 µM Vorinostat. Error bars show SEM. ***= p < 0.001, t-test. n = 3. d Cell survival of PEO1 and PEO4 cells on exposure to increasing concentrations of cisplatin for 24 h, following pre-treatment with vehicle, or 20 µM Vorinostat for 24 h. Error bars show SEM. n = 3.