Table 4 Correlation of NEFM transcriptional expression/DNA methylation with immunomodulators based on TISIDB and TCGA database

ADORA2A

0.137 (0.15)

5.07e−6 (2.5e−05)

− 0.095 (− 0.004)

0.00751 (0.26)

BTLA

0.032 (0.041)

0.296 (0.240)

− 0.477 (− 0.35)

2.2e−16

CD160

− 0.019 (0.07)

0.539 (0.046)

− 0.157 (− 0.15)

9.61e−06 (1.5e−05)

CD244

0.06 (0.076)

0.0467 (0.032)

− 0.525 (− 0.35)

 < 2.2e−16

CD274(PD-L1)

− 0.006 (0.038)

0.854 (0.28)

− 0.304 (− 0.28)

4.23e−18 (2.3e−15)

CD96

0.037 (0.042)

0.226 (0.23)

− 0.479 (− 0.34)

 < 2.2e−16

CSF1R

0.132 (0.11)

0.22e−05 (0.0027)

− 0.44 (− 0.34)

 < 2.2e−16

CTLA4

− 0.028 (0.00073)

0.357 (0.98)

− 0.432 (0.3)

 < 2.2e−16

HAVCR2

0.008 (− 0.0079)

0.789 (0.82)

− 0.298 (− 0.21)

2.16e−17 (1.8e−09)

IDO1

− 0.112 (− 0.1)

0.000203 (0.0036)

− 0.384 (− 0.26)

 < 2.2e−16 (1.2e−13)

IL10

− 0.003 (− 0.025)

0.921 (0.48)

− 0.388 (− 0.29)

 < 2.2e−16

IL10RB

− 0.08 (− 0.036)

0.00821 (0.31)

− 0.262 (− 0.2)

1.08e−13 (1.3e−08)

KDR(VEGFR)

0.127 (0.11)

2.51e−05 (0.0017)

− 0.117 (− 0.15)

0.000984 (2.9e−05)

LAG3

− 0.171 (− 0.14)

1.11e−08 (4.5e−05)

− 0.314 (− 0.2)

2.38e−19 (6.9e−09)

LGALS9

− 0.051 (− 0.059)

0.0912 (0.093)

− 0.293 (− 0.17)

9.15e−17 (1.2e−06)

PDCD1

0.007 (0.023)

0.816 (0.51)

− 0.43 (− 0.29)

 < 2.2e−16

PDCD1LG2

0.066 (0.076)

0.0281 (0.031)

− 0.476 (− 0.34)

 < 2.2e−16

PVRL2(NECTIN2)

0.027 (0.045)

0.378 (0.2)

0.32 (0.14)

2.9e−20 (9.9e−05)

TGFB1

0.32 (0.3)

 < 2.2e−16

− 0.28 (− 0.2)3)

1.91e−15 (3.6e−11)

TGFBR1

0.214 (0.21)

9.26e−13 (2.7e−09)

− 0.178 (− 0.13)

5.46e−07 (0.00014)

TGFBR2

(0.35)

 < 2.2e−16

(− 0.33)

 < 2.2e−16

TIGIT

− 0.02 (− 0.0034)

0.509 (0.92)

− 0.446 (− 0.31)

 < 2.2e−16

VTCN1

0.207 (0.21)

4.34e−12 (2.4e−09)

− 0.175 (− 0.07)

8.63e−07 (0.048)

C10orf54(VSIR, VISTA)

0.205 (0.18)

7.97e−12 (2.3e−07)

− 0.552 (− 0.39)

 < 2.2e−16

CD27(TNFRSF7)

0.097 ( (0.11)

0.00121 (0.0013)

− 0.452 (− 0.31)

 < 2.2e−16

CD276

0.168 (0.19)

2.12e−08 (9.7e−08)

− 0.038 (− 0.065)

0.284 (0.068)

CD28

0.042 (0.038)

0.16 (0.28)

− 0.458 (− 0.33)

 < 2.2e−16

CD40

0.137 (0.15)

5.1e−06 (2.2e−05)

− 0.553 (− 0.38)

 < 2.2e−16

CD40LG

0.088 (0.093)

0.00346 (0.0081)

− 0.457 (0.32)

 < 2.2e−16

CD48

0.062 (0.062)

0.0391 (0.079)

− 0.458 (− 0.31)

 < 2.2e−16

CD70

0.108 (0.11)

0.000345 (0.0017)

− 0.312 (− 0.17)

3.39e−19 (1.4e−06)

CD80

− 0.069

0.0228

− 0.259 (− 0.18)

1.95e−13 (2.8e−07)

CD86

− 0.028 (− 0.056)

0.357 (0.11)

− 0.375

 < 2.2e−16

CXCL12

0.467 (0.43)

 < 2.2e−16

− 0.32 (− 0.2)

3.31e−20 (1.5e−08)

CXCR4

0.053 (0.087)

0.0763(0.013)

− 0.313 (− 0.17)

3.11e−19 (8.2e−07)

ENTPD1(CD39)

0.267 (0.3)

2.85e−19 (< 2.2e−16)

− 0.271 (− 0.23)

1.46e−14 (5.5e−11)

ICOS

− 0.064 (− 0.044)

0.0341 (0.21)

− 0.42 (− 0.29)

 < 2.2e−16

ICOSLG

0.045 (0.0042)

0.15 (0.91)

− 0.193 (− 0.027)

5.69e−08 (0.44)

IL2RA

− 0.033 (− 0.022)

0.276 (0.53)

− 0.454 (− 0.33)

 < 2.2e−16

IL6

0.091 (0.087)

0.00266 (0.014)

− 0.425 (− 0.3)

 < 2.2e−16

IL6R

− 0.012 (− 0.015)

0.7 (0.67)

− 0.341 (− 0.29)

 < 2.2e−16

KLRC1

0.004 (0.017)

0.907 (0.64)

− 0.386 (− 0.28)

 < 2.2e−16 (2.7e−16)

KLRK1

0.041 (0.075)

0.171 (0.033)

− 0.456 (− 0.28)

 < 2.2e−16 (4.3e−16)

LTA

− 0.033 (− 0.015)

0.273 (0.66)

− 0.413 (− 0.28)

 < 2.2e−16 (1e−15)

MICB

0.003 (− 0.0094)

0.93 (0.79)

− 0.047 (− 0.16)

0.186(3e−06)

NT5E(CD73)

0.224 (0.2)

6.85e−14 (1e−08)

− 0.306 (− 0.28)

2.26e−18 (5.6e−16)

PVR

− 0.204 (− 0.17)

1.05e−11 (8.9e−07)

− 0.134 (− 0.11)

0.00017 (0.0019)

RAET1E

0.094 (0.12)

0.00182 (0.00065)

− 0.073 (− 0.016)

0.0411 (0.65)

TMEM173(STING)

0.231 (0.2)

1.14e−14 (6.5e−09)

− 0.263 (− 0.19)

9.54e−14 (1.1e−07)

TNFRSF13B

0.202 (0.21)

1.39e−11 (3.8e−09)

− 0.419 (− 0.31)

 < 2.2e−16

TNFRSF13C

0.068 (0.027)

0.0237 (0.44)

− 0.346 (− 0.26)

 < 2.2e−16 (4.6e−14)

TNFRSF14

0.048 (0.055)

0.109 (0.12)

− 0.074 (0.044)

0.0377 (0.21)

TNFRSF17

0.113 (0.13)

0.000181(0.00037)

− 0.396 (− 0.27)

 < 2.2e−16 (3.9e−15)

TNFRSF18

− 0.029 (− 0.04)

0.335 (0.25)

0.064 (− 0.021)

0.0727 (0.00037)

TNFRSF25

0.014 (0.078)

0.646 (0.028)

− 0.257 (− 0.14)

3.01e−13 (4.3e−05)

TNFRSF4

0.082 (0.082)

0.00664 (0.019)

− 0.281 (− 0.18)

1.51e−15 (2.9e−07)

TNFRSF8

0.086 (0.085)

0.00424 (0.015)

− 0.529 (− 0.37)

 < 2.2e−16

TNFRSF9

0.03 (0.036)

0.319 (0.3)

− 0.443 (− 0.36)

 < 2.2e−16

TNFSF13

− 0.015 (0.028)

0.617 (0.42)

0.047 (0.075)

0.189 (0.035)

TNFSF13B

− 0.015 (− 0.025)

0.623 (0.48)

− 0.343 (− 0.25)

 < 2.2e−16 (1.3e−12)

TNFSF14

0.082 (0.084)

0.00622 (0.017)

− 0.45 (− 0.32)

 < 2.2e−16

TNFSF15

0.095 (0.085)

0.00153 (0.015)

− 0.201 (− 0.12)

1.36e−08 (0.00091)

TNFSF4

0.149 (0.17)

6.77e−07 (2.1e−06)

− 0.081 (− 0.096)

0.0228 (0.0064)

TNFSF9

0.024 (0.086)

0.00165 (0.015)

− 0.256 (− 0.11)

3.82e−13 (0.0012)

ULBP1 (NKG2D)

− 0.029 (0.0054)

0.333 (0.88)

− 0.159 (− 0.015)

7.37e−06 (0.68)