Table 2 Comparison of costs between different sequencing-based methods for DNA methylation analyses
Bs-OS-Seq | Hybridization-based (e.g., SeqCap Epi) | Amplicon-based (e.g., QIAseq) | Whole-genome bisulfite sequencing (WGBS) | |
|---|---|---|---|---|
Wet bench protocol | Simple (resources: $) | Complex (resources: $$) | Complex (resources: $$) | Simple (resources: $) |
Sequencing adapters | Unmethylated ($) | Methylated ($$) | Unmethylated ($) | Methylated ($$) |
Sequencing cost | Targeted ($) | Targeted ($) | Targeted ($) | Whole-genome ($$$) |