Fig. 2

CpG methylation profiling of IL13 and ORMDL3 genes' islands and shores and comparison of bs-OS-Seq and 450k array in PBMC from healthy males. a DNA methylation in 6 kb of CpG islands and shores were profiled using bs-OS-Seq. Negative (un-methylated) and positive (fully methylated) reference DNA extracted from whole blood of NA12878 demonstrate consistent quantitativity of the assay. Six PBMC samples from six healthy male donors and the reference sample NA12878 show distinct hypomethylation of the CpG island while having varying degree of methylation in the CpG sites residing in the shores. b bs-OS-Seq demonstrated 19x and 15x higher resolution of CpG methylation compared to 450k array data in the PBMC samples in IL13 (268 vs. 14 CpG sites) and ORMDL3 (259 vs. 17 CpG sites) genes, respectively, while reaching concordant methylation results in the shared sites. c The 10% most variable CpG sites contained large variation between the PBMC samples from the six healthy male donors. CpG sites also covered by the 450 K array assay are highlighted with a green box