Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function

Clinical Epigenetics

Table 3 Semen characteristics and chromatin compaction status of fertile and infertile men

Group	% Progressive Motility	Concentration (× 10⁶)/ml	% Viability	% Aniline blue positive sperm	% CMA3 positive sperm
Fertile* (n = 14)	64 ± 10.4	43 ± 24.3	80 ± 7.5	17 ± 11.3	23 ± 11
Infertile
Asthenozoospermia (n = 10)	14 ± 6.9^a	18 ± 7.4^ab	69 ± 8.7^a	69 ± 11.2^a	70 ± 16.3^a
Oligozoospermia (n = 10)	43 ± 8.2^ab	6 ± 2.7^a	75 ± 8.0^b	58 ± 16.2^a	67 ± 19.3^a
Oligoasthenozoospermia (n = 10)	17 ± 7.5^a	4 ± 2.0^a	60 ± 11.7^a	69 ± 16.6^a	77 ± 16.6^a

*Fertile group (n = 14) encompasses men enrolled for the experiments involving ChIP-qPCR (n = 4) and qRT-PCR (n = 10)
^aSignificantly different as compared to fertile group
^bSignificantly different as compared to oligoasthenozoospermia group

ISSN: 1868-7083