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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function

Fig. 6

Chromatin condensation in sperm of fertile and infertile men: Histone status of sperm was assessed by Aniline blue staining. Representative picture showing darkly stained immature sperm nuclei and light stained mature nuclei in fertile and infertile semen sample (a-left panel). Protamine status was assessed by Chromomycin A3 staining. Representative picture of sperm from fertile and infertile semen samples shows bright fluorescing sperm nuclei which are CMA3 positive and dull fluorescing which are CMA3 negative (b-left panel). Dot plot shows percentage of aniline blue (a-right panel) and CMA3 positive sperm (b-right panel) in the fertile (n = 18) and infertile individuals, namely, men with Asthenozoospermia (n = 10), Oligoasthenozoospermia (n = 10) and Oligozoospermia (n = 10); p**** < 0.0001

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