Table 2 Schematic overview of the diagnostic analyses performed for determining molecular biomarkers according to WHO 2016 classification
From: DNA methylation profiling for molecular classification of adult diffuse lower-grade gliomas
Molecular diagnostic biomarkers | Molecular techniques used in clinical diagnostics | Methylation profiling |
|---|---|---|
Tumor grade | Histology | – |
IDHa mutation status | Immunohistochemistry | G-CIMPc |
Next-generation sequencing | ||
Sanger sequencing | ||
1p/19q codel.b | Fluorescent in situ hybridization Multiplex ligation-dependent probe amplification | Copy number variation profiles |