Fig. 3

Validation of the dual-marker detection model. a Differential methylation levels of the dual markers between non-BC and BC groups in Cohort 2; the methylation levels were represented reversely by ∆Ct of each marker; the predicted status was made by the assay of dual-marker model. b ROC curves of dual-marker detection model in Cohort 2. c Distributions of dual-marker model BC risk probability between non-BC and BC groups in Cohort 2; unpaired t test was used to analyze statistical significance; ****p < 0.0001. d Differential methylation levels of the dual markers between Non-BC and BC groups in Cohort 3; the methylation levels were represented reversely by ∆Ct of each marker; the predicted status was made by the assay of dual-marker model. e ROC curves of dual-marker detection model in Cohort 3. f Distributions of dual-marker model BC risk probability between non-BC and BC groups in Cohort 3; unpaired t test was used to analyze statistical significance; ****p < 0.0001