Fig. 3

The impact of CPEB1 overexpression on colorectal cancer cell lines in vitro. a Expression of CPEB1 was evaluated in SW480 and HCT116 cells by qPCR with the expression of ACTB (encoding β-actin) used as an internal reference. Control, untransfected CRC cell lines; Empty, CRC cells transfected with the pcDNA3.1 vector alone; CPEB1, CRC cells transfected with the pcDNA3.1-CPEB1 recombinant expression vector. Levels of CBEP1/β-Actin mRNA presented are shown as relative CBEP1 mRNA levels (2^−∆∆Ct); those in the control group were normalised to 1; ***P < 0.001. b Immunoreactive CPEB1 was detected in SW480 and HCT116 cells by WB, with GAPDH used as an internal control. CBEP1/GAPDH are presented as relative protein intensity; values in the control group were normalised to 1. Data are presented as the mean ± SD for three independent experiments. c, d Proliferation of SW480 and HCT116 cells detected by CCK-8 assay at an OD 450 nm. e An in vitro scratch/wound healing assay that evaluated relative wound width over time as a measure of cellular migration. f CRC proliferation and growth were determined by a colony-forming assay. g CPEB1 overexpression in SW480 and HCT116 cells reduced their capacity for migration and invasion in a transwell assay. h) CPEB1 overexpression in SW480 and HCT116 cells resulted in a significant increase in the rate of cellular apoptosis. Data are presented as the mean ± SD for three independent experiments; **P < 0.01; ***P < 0.001