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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Identification of ZBTB18 as a novel colorectal tumor suppressor gene through genome-wide promoter hypermethylation analysis

Fig. 3

Effects of ZBTB18 overexpression on the growth of colon cancer cells. The effects of ZBTB18 overexpression on the growth of HCT116 cells was assessed by directly counting the number of cells in culture over time (a). The growth of HCT116 cells was monitored over time after subcutaneous implantation in immunodeficient NOD/SCID mice (b). At the end of the experiment the tumors were dissected out (c) and the average weight (± SEM) of the tumors was measured (d). Cells in the different phases of the cell cycle (e) and SubG1 cells (f) were assessed to evaluate the effect of ZBTB18 overexpression in cell growth and apoptosis. Migration (g) and invasion (h) properties of parental and ZBTB18 overexpressing cells were assessed in wound healing and Matrigel-coated Boyden chamber assays, respectively. Student’s t test *p < 0.05, **p < 0.01 and ***p < 0.001

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Clinical Epigenetics

ISSN: 1868-7083

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