Fig. 7

miR-503-5p inhibition or CXCL10 elevation negates HDAC2 knockout-induced effects on ESCC tumorigenesis. a RT-qPCR to detect CXCL10 mRNA expression in ESCC cells; b Western blot assay to detect CXCL10 protein expression in ESCC cells; c MTT assay to detect ESCC cell viability; d colony formation assay to detect ESCC cell colony-forming ability; e flow cytometry to detect ESCC cell apoptosis rate; f transwell assay to detect ESCC cell migration ability; g transwell assay to detect ESCC cell invasion ability; for all quantitative results, the data were expressed as mean ± standard deviation of three independent experiments. Discrepancies among multiple groups were assessed by one-way ANOVA;* P < 0.05 compared with the sh-HDAC2 group