Fig. 2

Silencing HDAC2 depresses ESCC cell viability and colony-forming ability, and enhances apoptosis. a RT-qPCR to detect HDAC2 mRNA expression in ESCC cells; b MTT assay to detect ESCC cell viability; c colony formation assay to detect ESCC cell colony-forming abilities; d flow cytometry to detect ESCC cell apoptosis rate; e RT-qPCR to detect E-cadherin and Vimentin protein expression in ESCC cells; for all quantitative results, the data were expressed as mean ± standard deviation of three independent experiments. Discrepancies among multiple groups were assessed by one-way ANOVA; * P < 0.05 compared with the sh-NC group