Fig. 2

Biological or chemical inhibition of HER2 modulate the expression of CBP in HER2-overexpressing cells. a, b Upper panel: Representative western blot images for HER2 and CBP expression in a SkBr3 and b BT-474 cells transfected with negative control (NC) or HER2 siRNA for 24–96 h. Lower panel: Relative densitometric graphs of CBP expression after normalization to β-actin. Fold change of CBP expression was done over cells transfected with NC siRNA for each time point. c, d Upper panel: Images for immunoblots of CBP in c SkBr3 and d BT-474 cells treated with 10 μg/mL of HER2-specific humanized antibody Trastuzumab or with DMSO (control cells) for 24–96 h. Lower panel: Graphs for relative expression level of CBP after normalization to β-actin. Full blots images are available in Additional file 1: Fig. S5 and Additional file 1: Fig. S6. Average present as mean ± SEM (n = 3). *p < 0.05 vs untreated control, unpaired t test