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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Integrative study of EZH2 mutational status, copy number, protein expression and H3K27 trimethylation in AML/MDS patients

Fig. 3

EZH2 expression and H3K27 trimethylation. a Representative example of strongly positive H3K27me3 expression in BM cells from a patient. b Representative example of low H3K27me3 expression in BM cells from a control case. c Linear regression analysis of 31 EZH2-wt cases shows an association between EZH2 expression and H3K27me3 levels, even though H3K27me3 levels are highly variable (R2 = 0.2, p = 0.01). d In 9 EZH2-mut cases, no association between EZH2 expression and H3K27me3 levels could be detected by linear regression analysis (R2 = 0.12, p = 0.36). e Western blot on EZH2 mutant and wild-type AML cell lines and AML patient samples. Western blot results for EZH2 and H3K27me3 using whole cell extracts from EZH2 mutant samples (mut; AML cell line SKM-1 and three AML patient samples) and EZH2 wild-type samples (wt; APL cell line NB-4 and two AML patient samples). All patient data can be found in Tables 2 and 3. Beta-actin was used as loading control. Space between bands was cropped to save space

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