Fig. 1

Pharmacological inhibition by VPA and SAHA caused broad acetylation and deacetylation patterns. a Chemical structures of VPA and SAHA. b Plan of experiments and analyses performed using chromatin immunoprecipitation (ChIP) using antibodies that specifically recognise H3K9/14ac, H3K4me3 and H3K9me3 and gene expression using massive parallel sequencing and mapping. ChIP-Seq was performed from five experiments and RNA-Seq from three experiments for the untreated control and cells exposed to VPA and SAHA. c ChIP followed by sequencing (ChIP-seq) shows MACS (Bonferroni-adjusted P < 0.05) derived bipartite acetylation (elevated H3K9/14ac in yellow) and deacetylation peaks (reduced H3K9/14ac in green) in cells stimulated with VPA. d ChIP-seq shows MACS (Bonferroni-adjusted P < 0.05) derived acetylation (elevated H3K9/14ac in yellow) and deacetylation peaks (reduced H3K9/14ac in green) in cells stimulated with SAHA