Fig. 5

DNA and CpG island locations throughout the MGMT gene region (NM_002412; Chr10: 131,265,478—131,265,604). The CpG are represented as circles. The green circles symbolize the critical CpGs described by Malley et al. The orange circle symbolizes the cg12981137 described by Bady et al. The red and blue arrows represent M and U primers respectively. Between the primers, the hydrolysis probes labeled with two different reporter fluorochromes specific for recognizing methylated or Unmethylated DNA