Fig. 6

Immunofluorescence (IF) staining of selected candidates in control and HCM hiPSC-cardiomyocytes. The same amount of control and HCM hiPSC-cardiomyocytes (20,000 cells per well) were seeded to the plate and cultured for 27 days prior to the staining. a Representative IF staining showing the expression of KLF15 in HCM and control cardiomyocytes (KLF15: red; ACTN2: green; blue: cell nuclei). b Representative IF staining showing disrupted ACTN2 in HCM cardiomyocytes compared to controls (ACTN2: green; blue: cell nuclei). c Representative IF staining showing suppressed ATP2A2 in HCM cardiomyocytes compared to controls (ATP2A2: red; blue: cell nuclei). Nuclei were stained by Hoechst. All overview images were taken at 20 × magnification, scale bar = 100 µm. All zoomed regions were taken under Zoom Factor = 1.5