Fig. 5

Chronic DZNep treatment reduces EZH2 and H3K27me3 levels while reprogramming the transcriptome in tumors induced by NNK in the A/J mouse lung. Western blot showing the levels of EZH2, β-actin, H3K27me3 and total histone H3 in tumors from vehicle and DZNep treated mice (a). Densitometry quantitation of EZH2 and H3K27me3 levels normalized to β-Actin and total histone H3 (b, c). Values are mean ± SEM from n = 10/group. *p < 0.05 compared to normal. Venn diagrams showing common genes (middle) with differential expression in tumors comparing DZNep to EPZ6438 + UNC0642 or Lovaza treatment (d). RT-qPCR for expression of GBX2, IL10RA, SOX9, and HOXA5 in tumors from vehicle and DZNep treated mice (e). Horizontal line within the boxes reflects the median and the whisker indicate the range (min to max) for relative levels of expression normalized to β-Actin. *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle (n = 3)