Fig. 2

Perpetuation and progression of epigenetic changes in tumors and chromatin remodeling in HBECT. Flow diagram depicting the number of genes methylated in transformed HBEC lines that are also methylated in tumor lines and TCGA tumors (a). Combined bisulfite restriction analysis comparing methylation of SURF, PELI1, FEZ1, GPD2, and IGSF9 between HBEC2T and the tumor lines H1975, A549, H2009, and Calu6 (b). Flow diagram depicting the number of unmethylated genes with reduced expression in transformed HBECs that become methylated in tumor lines and TCGA tumors or retain reduced expression in tumors (c). Chromatin immunoprecipitation showing enrichment of H3K27me3 (d) or H3K9me2 (e) in HBEC2T relative to HBEC2 for 11 genes listed on the x-axis. Values are mean ± SEM for ChIP-qPCR conducted in triplicate