Skip to main content
Fig. 4 | Clinical Epigenetics

Fig. 4

From: BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer

Fig. 4

BRG1 regulates genes involved the cell cycle. a Gene set enrichment analysis using ‘Enrichr’ of down-regulated genes at 144 h post BRG1 knockdown. Enriched GO terms are classified as biological processes, cellular component or molecular function. Adjusted p value (log 10, reversed x-axis) of the 10 most significant GO terms is shown. b Heatmap of gene expression profiles from the top 40 differential cell cycle genes after BRG1 depletion. Expression is shown as the normalised row Z-score of transcripts per million reads (TPM), with blue indicating higher expression and red indicating lower expression. Genes involved in DNA replication initiation are indicated in blue. c, d Gene expression from RNA-seq (TPM), of the MCM2-7 helicase components (top) and the origin of replication complex (ORC) subunits (bottom). Bars denote mean, and error bars are SD. e Representative Western blot showing protein levels of replication initiation genes MCM5, CDC6 and ORC6, along with CHK1, after 72 and 144 h post BRG1 depletion. f Quantification of Western blots demonstrating adjusted relative density to GAPDH (n = 2). Error bars are SD

Back to article page
\