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Fig. 3 | Clinical Epigenetics

Fig. 3

From: BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer

Fig. 3

BRG1 regulates genes associated with prostate cancer. a Gene set enrichment analysis using ‘Enrichr’ of differentially expressed genes that are common to both time points, showing the adjusted p value (log 10, reversed x-axis) of significantly enriched transcription factor ChIP-seq from ChEA curated data (p < 0.05). b Heatmap of BRG1, AR and FOXA1 ChIP-seq signal at BRG1 binding sites genome-wide in LNCaP cells, ± 2.5 kb from the centre of the binding site. Data are clustered into three groups by k-means. c IGV images of the genes KLK2, PCAT-1 and VAV3. Grey shaded regions contain ChIP-seq signal peaks for BRG1, AR and FOXA1. d Representative Western blot of the abundance of soluble unbound proteins versus chromatin bound in control cells and 144 h post BRG1 depletion (n = 3). Vinculin served as the soluble unbound control and H2A as the chromatin bound control. e Representative Western blot of co-IPs for BRG1, AR, FOXA1 and IgG control, alongside the supernatant (unbound fraction) and input representing 1% of the total protein in each sample (n = 3). Samples were collected in basal cell culture conditions in control cells

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