Fig. 5
NOX4 upregulated SNAIL1 expression by promoting histone methylation. a The protein expression of histone methylation markers after NOX4 overexpression. b The expression of H3K4ME3 and the quantification of signal intensities after NOX4 overexpression. Bar = 20 μm. c The protein expression of histone methylation markers after NOX4 knockdown with or without of 1%O2 treatment. d The expression of H3K4ME3 and the quantification of signal intensities after NOX4 knockdown with or without of 1%O2 treatment. Bar = 20 μm. e, f The mRNA level of SNAIL1 in HPAC and Panc1 cells after overexpression and knockdown of NOX4 with or without of 1%O2 treatment. g, h ChIP-qPCR analysis of H3K4me3 for SNAIL1 in HPAC and Panc1 cells after overexpression and knockdown of NOX4 with or without of 1%O2 treatment. *p < 0.05, **p < 0.01, ***p < 0.001