Fig. 2
EMT induced by hypoxia in pancreatic cancer cells was dependent on NOX4 expression. a The heat map showed Pearson’s correlation analyses between hypoxia score and NADPH oxidase. b The mRNA and c protein expression of NOX4 after time gradient of hypoxia treatment in HPAC and Panc1 cells. d The protein expression of CDH1, CDH2, VIM and SNAIL1 after the concentration gradient of H2 O2 treatment. e The protein expression of CDH1, CDH2, VIM and SNAIL1 after NOX4 overexpression. f The expression and quantification of signal intensities of NOX4, VIM, CDH2 in HPAC cells after NOX4 overexpression were determined by immunofluorescence. Bar = 20 μm. g The protein expression of CDH1, CDH2, VIM and SNAIL1 after NOX4 knockdown and hypoxia treatment. h The expression and quantification of signal intensities of NOX4, VIM, CDH2 in HPAC cells after NOX4 knockdown and hypoxia treatment were determined by immunofluorescence. Bar = 20 μm. ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001