Fig. 1
Hypoxia-related gene expression was enriched in a subset of pancreatic cancer samples and correlated with EMT pathway. a The volcano diagrams showed differentially expressed mRNAs between pancreatic cancer tissue and normal tissue samples from GSE15471 (left panel) and GSE16515 (right panel). Hypoxia-related genes were highlighted using gene name. b Venn diagram showed that 30 hypoxia-related genes were both upregulated in tumor tissues in 2 databases. c Overall survival and progression-free survival between the high hypoxia score group and low hypoxia score group. d Pie charts showing proportions of the clinical parameters and molecular subtypes between high hypoxia score group and low hypoxia score group in samples of pancreatic cancer from TCGA. e The heat map showed the top10 gene sets after GSVA analysis. f Pearson’s correlation analyses between hypoxia score and EMT score. g The mRNA and h protein expression of EMT markers after 1%O2 treatment for 24 h were assessed by qRT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001