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Table 1 Main glycans on secreted glycoproteins in all cell lines

From: 5-AZA-dC induces epigenetic changes associated with modified glycosylation of secreted glycoproteins and increased EMT and migration in chemo-sensitive cancer cells

Peak GU Major glycans
A2780 A2780cis PEO1 PEO4 MDA-MB-231 MDA-MB-436
1 6.37 FA1G1 FA2 FA1G1 FA2 M5 FA2
2 6.47 M5 M5 M5 A2G1/FA2[6]G1 A1G1S[6]1 M5
3 6.72 A2F1G1 A2F1G1 FA2[3]G1/A2F1G1 FA2[3]G1 A1G1S[6]1 A2F1G1
4 7.19 A2F1G1 A2F1G1 A2F1G1 A2F1G1 A2F1G1 A2F1G1
5 7.34 A2F1G1 A2F1G1 A2F1G1 A2F1G1 A2F1G1 A2F1G1
6 7.44 FA3G1 FA3G1 FA3G1 A2F1G1 M6 M6/FA3G1
7 7.58 M5A1G1/FA2G2 M5A1G1/FA2G2 M5A1G1 M5A1G1 M5A1G1/FA2G2 FA2G2
8 8.20 FA2BG2/FA3G2 FA2BG2/FA3G2 FA3G2 FA2BG2/FA3G2 FA2BG2/FA3G2 FA2BG2/FA3G2
9 8.47 M7/M4A1G1S1 M7/M4A1G1S1 M4A1G1S1 M7 M4A1G1S1 M7
10 8.62 A2G2S1 A2G2S1 A2G2S1 A2G2S1/A3G1S1 A2G2S1 A2G2S1/A3G1S1
11 8.97 A2G2S1 A2G2S1 A2G2S1 A3G3 A2G2S1 A2G2S1
12 9.13 M8 M8 M8 FA2F2G2 FA2F2G2 M8/FA2G2S1
13 9.45 M5A2BF1G2Lac1S(6)1 M5A2BF1G2Lac1S(6)1 M5A2BF1G2Lac1S(6)1 M5A2BF1G2Lac1S(6)1 M5A2BF1G2Lac1S(6)1 M5A2BF1G2Lac1S(6)1/FA2F2G2/FA2G1Lac1S1
14 9.86 A2G2S2 A2G2S2 A2G2S2 A3G2S1 A2G2S2 FA3′G3
15 9.99 A2G2S2 A2G2S2 A2G2S2 A2G2S2 A2G2S2 A2G2S2
16 10.16 FA2G2S2 FA2G2S2 FA2G2S2 FA2G2S2 FA2G2S2 FA2G2S2
17 10.32 A2G2S2 A2G2S2 A2G2S2 A2G2S2 A2G2S2 A2G2S2
18 10.47 A2G2S2 FA3G2S1 FA3G2S1 FA3G2S1/FA2F2G2S1 FA3G2S1 A4G4
19 10.64 FA2G2S2 FA2G2S2 FA2G2S2 FA2G2S2 FA2G2S2 FA2G2S2
20 10.81 A3G3S2 A3G3S2 A3G3S2 A3G3S2 A3G3S2 A3G3S2
21 10.98 A2G2S2/FA2G2S2/A2BG2S2 A2G2S2/FA2G2S2/A2BG2S2 A2G2S2/FA2G2S2/A2BG2S2 A2G2S2/A2BG2S2 A2BG2S2/A3F2G3 A3G3S1/FA2G2S2/A2BG2S2/A3F2G3
22 11.25 A3G3S2 A3G3S2 A3G3S2 A3G3S2 A3G3S2 A3G3S2
23 11.42 A3G3S2/FA3BG3S1 A3G3S2/FA3BG3S1 A3G3S2/FA3BG3S1 A3G3S2/A3F1G2S1 A3G3S2/FA3BG3S1 A3G3S2/A3F1G2S1
24 11.72 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3
25 11.97 A3G3S2 A3G3S2 A3G3S2 A3G3S2 A3G3S2 A3G3S2
26 12.15 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3
27 12.31 A3F1G3KDN3 A3F1G3KDN3 A3F1G3KDN3 A3F1G3KDN3 A3F1G3KDN3 A3F1G3KDN3
28 12.96 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3
29 12.58 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3
30 12.76 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3
31 13.04 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3 A3G3S3
32 13.44 A3G3S4 A3G3S4 A3G3S4 A3G3S4 M5A2G2S2 A3G3S4
33 13.62 FA3BG3S4 FA3BG3S4/FA4F1G1S2 FA3BG3S4 FA3BG3S4/A3F2G3S3 FA3BG3S4/FA4F1G1S2 FA4F1G1S2
34 13.96 A2G3Lac1S2 A2G3Lac1S2 A2G3Lac1S2 A2G3Lac1S2 A2G3Lac1S2 A2G3Lac1S2
35 14.18 FA3′BG3S(3)4 FA3′BG3S(3)4 FA3′BG3S(3)4 FA3′BG3S(3)4 FA3′BG3S(3)4 FA3′BG3S(3)4
  1. All N-glycans have two core GlcNAcs; F, at the start of the abbreviation indicates a core-fucose α1,6-linked to the inner GlcNAc; Mx, number (x) of mannose on core GlcNAcs; D1, indicates that the α1-2 mannose is on the Manα1-6Manα1-6 arm, D2, on the Manα1-3Manα1-6 arm, D3, on the Manα1-3 arm of M6 and on the Manα1-2Manα1-3 arm of M7 and M8; Ax, the number of antenna (GlcNAc) on trimannosyl core; A2, biantennary with both GlcNAcs as β1,2-linked; A3, triantennary with a GlcNAc linked β1,2 to both mannose and the third GlcNAc linked β1,4 to the α1,3 linked mannose; A4, GlcNAcs linked as A3 with additional GlcNAc β1,6 linked to α1,6 mannose; B, bisecting GlcNAc linked β1,4 to β1,3 mannose; Gx, number (x) of β1,4 linked galactose on antenna; F(x), number (x) of fucose linked α1,3 to antenna GlcNAc; Sx, the number (x) of sialic acids linked to galactose. Where not specified, sialic acid is linked both α2,3 and α2,6
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