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Table 1 Main glycans on secreted glycoproteins in all cell lines

From: 5-AZA-dC induces epigenetic changes associated with modified glycosylation of secreted glycoproteins and increased EMT and migration in chemo-sensitive cancer cells

Peak

GU

Major glycans

A2780

A2780cis

PEO1

PEO4

MDA-MB-231

MDA-MB-436

1

6.37

FA1G1

FA2

FA1G1

FA2

M5

FA2

2

6.47

M5

M5

M5

A2G1/FA2[6]G1

A1G1S[6]1

M5

3

6.72

A2F1G1

A2F1G1

FA2[3]G1/A2F1G1

FA2[3]G1

A1G1S[6]1

A2F1G1

4

7.19

A2F1G1

A2F1G1

A2F1G1

A2F1G1

A2F1G1

A2F1G1

5

7.34

A2F1G1

A2F1G1

A2F1G1

A2F1G1

A2F1G1

A2F1G1

6

7.44

FA3G1

FA3G1

FA3G1

A2F1G1

M6

M6/FA3G1

7

7.58

M5A1G1/FA2G2

M5A1G1/FA2G2

M5A1G1

M5A1G1

M5A1G1/FA2G2

FA2G2

8

8.20

FA2BG2/FA3G2

FA2BG2/FA3G2

FA3G2

FA2BG2/FA3G2

FA2BG2/FA3G2

FA2BG2/FA3G2

9

8.47

M7/M4A1G1S1

M7/M4A1G1S1

M4A1G1S1

M7

M4A1G1S1

M7

10

8.62

A2G2S1

A2G2S1

A2G2S1

A2G2S1/A3G1S1

A2G2S1

A2G2S1/A3G1S1

11

8.97

A2G2S1

A2G2S1

A2G2S1

A3G3

A2G2S1

A2G2S1

12

9.13

M8

M8

M8

FA2F2G2

FA2F2G2

M8/FA2G2S1

13

9.45

M5A2BF1G2Lac1S(6)1

M5A2BF1G2Lac1S(6)1

M5A2BF1G2Lac1S(6)1

M5A2BF1G2Lac1S(6)1

M5A2BF1G2Lac1S(6)1

M5A2BF1G2Lac1S(6)1/FA2F2G2/FA2G1Lac1S1

14

9.86

A2G2S2

A2G2S2

A2G2S2

A3G2S1

A2G2S2

FA3′G3

15

9.99

A2G2S2

A2G2S2

A2G2S2

A2G2S2

A2G2S2

A2G2S2

16

10.16

FA2G2S2

FA2G2S2

FA2G2S2

FA2G2S2

FA2G2S2

FA2G2S2

17

10.32

A2G2S2

A2G2S2

A2G2S2

A2G2S2

A2G2S2

A2G2S2

18

10.47

A2G2S2

FA3G2S1

FA3G2S1

FA3G2S1/FA2F2G2S1

FA3G2S1

A4G4

19

10.64

FA2G2S2

FA2G2S2

FA2G2S2

FA2G2S2

FA2G2S2

FA2G2S2

20

10.81

A3G3S2

A3G3S2

A3G3S2

A3G3S2

A3G3S2

A3G3S2

21

10.98

A2G2S2/FA2G2S2/A2BG2S2

A2G2S2/FA2G2S2/A2BG2S2

A2G2S2/FA2G2S2/A2BG2S2

A2G2S2/A2BG2S2

A2BG2S2/A3F2G3

A3G3S1/FA2G2S2/A2BG2S2/A3F2G3

22

11.25

A3G3S2

A3G3S2

A3G3S2

A3G3S2

A3G3S2

A3G3S2

23

11.42

A3G3S2/FA3BG3S1

A3G3S2/FA3BG3S1

A3G3S2/FA3BG3S1

A3G3S2/A3F1G2S1

A3G3S2/FA3BG3S1

A3G3S2/A3F1G2S1

24

11.72

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

25

11.97

A3G3S2

A3G3S2

A3G3S2

A3G3S2

A3G3S2

A3G3S2

26

12.15

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

27

12.31

A3F1G3KDN3

A3F1G3KDN3

A3F1G3KDN3

A3F1G3KDN3

A3F1G3KDN3

A3F1G3KDN3

28

12.96

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

29

12.58

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

30

12.76

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

31

13.04

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

A3G3S3

32

13.44

A3G3S4

A3G3S4

A3G3S4

A3G3S4

M5A2G2S2

A3G3S4

33

13.62

FA3BG3S4

FA3BG3S4/FA4F1G1S2

FA3BG3S4

FA3BG3S4/A3F2G3S3

FA3BG3S4/FA4F1G1S2

FA4F1G1S2

34

13.96

A2G3Lac1S2

A2G3Lac1S2

A2G3Lac1S2

A2G3Lac1S2

A2G3Lac1S2

A2G3Lac1S2

35

14.18

FA3′BG3S(3)4

FA3′BG3S(3)4

FA3′BG3S(3)4

FA3′BG3S(3)4

FA3′BG3S(3)4

FA3′BG3S(3)4

  1. All N-glycans have two core GlcNAcs; F, at the start of the abbreviation indicates a core-fucose α1,6-linked to the inner GlcNAc; Mx, number (x) of mannose on core GlcNAcs; D1, indicates that the α1-2 mannose is on the Manα1-6Manα1-6 arm, D2, on the Manα1-3Manα1-6 arm, D3, on the Manα1-3 arm of M6 and on the Manα1-2Manα1-3 arm of M7 and M8; Ax, the number of antenna (GlcNAc) on trimannosyl core; A2, biantennary with both GlcNAcs as β1,2-linked; A3, triantennary with a GlcNAc linked β1,2 to both mannose and the third GlcNAc linked β1,4 to the α1,3 linked mannose; A4, GlcNAcs linked as A3 with additional GlcNAc β1,6 linked to α1,6 mannose; B, bisecting GlcNAc linked β1,4 to β1,3 mannose; Gx, number (x) of β1,4 linked galactose on antenna; F(x), number (x) of fucose linked α1,3 to antenna GlcNAc; Sx, the number (x) of sialic acids linked to galactose. Where not specified, sialic acid is linked both α2,3 and α2,6
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Clinical Epigenetics

ISSN: 1868-7083

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