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Fig. 6 | Clinical Epigenetics

Fig. 6

From: 5-AZA-dC induces epigenetic changes associated with modified glycosylation of secreted glycoproteins and increased EMT and migration in chemo-sensitive cancer cells

Fig. 6

Expression of GATA2 and 3 affects the expression of MGAT5 and ST3GAL4. a Quantitative RT-PCR analysis of MGAT5 and ST3GAL4 and their transcription factors (GATA2 and GATA3). The relative mRNA expression level of each gene was calculated according to the ddCt method, normalised to TBP. Each condition represents two biological replicates. b Densitometric (Image J) fold change of the protein expression of GATA2, GATA3, MGAT5 and ST3GAL4 following siRNA knockdown of GATA2 or 3 (Western blots are in Additional file 5: Figure S7). Each condition represents two or three biological replicates. *p value  ≤ 0.05 or **p value ≤ 0.005 (fold expression in particular conditions was compared as follows: untreated vs 5-AZA-dC treated and both conditions with vs without siGATA2 treatments). (Two-way ANOVA and Spearman’s correlation) All p values for this figure are in Additional file 4: Table S3 including the correlation between mRNA and protein expression)

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