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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Identification and validation of obesity-related gene LEP methylation as a prognostic indicator in patients with acute myeloid leukemia

Fig. 2

Validation and confirmation of LEP methylation in AML. a The genomic coordinates (GC) of LEP promoter region CpG island and primer locations. The panel plots the GC content as a percentage of the total. Each vertical bar in the bottom panel represents the presence of a CpG dinucleotide. Black horizontal lines indicate regions amplified by sequencing primer pairs and qMSP primer pairs. CpGplot (http://emboss.bioinformatics.nl/cgi-bin/emboss/cpgplot) and Methyl Primer Express v1.0 software were used for creating the figure. TSS transcription start site, qMSP real-time quantitative methylation-specific PCR; b LEP methylation level in controls and AML patients detected by targeted bisulfite sequencing. c Correlation between targeted bisulfite sequencing and qMSP results for LEP methylation in AML patients. The correlation analysis was conducted by Spearman test. d LEP methylation level in controls and AML patients examined by qMSP. AML included de novo AML and sAML which indicated MDS-derived AML. e LEP expression level in controls and AML patients. LEP expression level was examined by qPCR. f ROC curve analysis of LEP methylation distinguishing AML from controls

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